The incubation time in liquid paraffin should be lengthy enough to get suitable infiltration, but not any more than essential to be able to prevent extended exposure on the tissue to heat. For the same explanation, it is usually recommended the temperature with the liquid paraffin really should be not more than two–four C above the paraffin's mel
That is especially legitimate for immunohistochemistry in which ten% NBF has become demonstrated being a lousy fixative (Fig. two). Some issues with using ten% NBF as a fixative are actually corrected with the introduction of other experimental procedures for instance antigen retrieval (Shi et al. 2001) and the usage of modest amplicons in RT-Q-PCR
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